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KMID : 0861020120270060115
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Korea Journal of Herbology
2012 Volume.27 No. 6 p.115 ~ p.122
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Comparative study on efficacies and ingredient contents of different solvent extracts of Insampaedok-san
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Lim Hye-Sun
Ha Hye-Kyung
Seo Chang-Seob
Jin Seong-Eun
Kim Ye-Ji
Jeon Woo-Young
Yoo Sae-Room
Shin In-Sik
Kim Jung-Hoon
Kim Seong-Sil
Shin Na-Ra
Lee Mee-Young
Jeong Soo-Jin
Kim Ohn-Soon
Shin Hyeun-Kyoo
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Abstract
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Objectives: This study evaluated activities and ingredient contents concerning extracts according to extraction
solvents of Insampaedok-san (IS, Renshen bai du-san).
Methods: The herbal constituents of IS were extracted with water and 70% ethanol at 100°C for 2 hr. Using the HPLC system, the six ingredient contents of different solvent extracts of IS were analyzed. The nitric oxide (NO), prostaglandin E2 (PGE2) production and proinflammatory cytokines were measured in RAW264.7 cells stimulated with lipopolysaccharide (LPS). The macrophage-derived chemokine (MDC/CCL22) and regulated on activation normal T-cell expression and secreted (RANTES/CCL5) production were measured in HaCaT and BEAS-2B cells stimulated tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). The activities of glycerol-3-phosphate dehydrogenase (GPDH) and leptin level were measured in 3T3-L1 cells.
Results: The calibration curves showed good linearity (r2=1.0000) for different concentration ranges. The contents of liquiritin, naringin, hesperidin, neohesperirin and glycyrrizin in 70% ethanol extracts of IS were relatively higher than that of water extract, however the content of ferulic acid in 70% ethanol and water extract of IS were similar. The extraction solvents of water and 70% ethanol were evaluated inhibitory effect on the production of NO, PGE2, TNF-α and IL-6 in RAW 264.7 cells. Their extractions were inhibitory effect on production of MDC/CCL22 and RANTES/CCL5 in HaCaT cell and BEAS-2B cell, respectively. In addition, evaluated reduced on GPDH activity and leptin level in 3T3-L1 preadipocyte cell.
Conclusions: Our results suggest that IS extracts were inhibitory effects of disease such as inflammation, allergies and obesity.
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KEYWORD
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Insampaedok-san (Renshen bai du-san), anti-inflammation, anti-allergy, anti-obesity
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